Optical biosensor technologies for molecular diagnostics at the point-of-care

Joerg Schotter; Stefan Schrittwieser; Paul Muellner; Eva Melnik; Rainer Hainberger; Guenther Koppitsch; Franz Schrank; Katerina Soulantika; Sergio Lentijo-Mozo; Beatriz Pelaz; Wolfgang Parak; Frank Ludwig; Jan Dieckhoff

doi:10.1117/12.2185642

13 May 2015 Optical biosensor technologies for molecular diagnostics at the point-of-care

Joerg Schotter, Stefan Schrittwieser, Paul Muellner, Eva Melnik, Rainer Hainberger, Guenther Koppitsch, Franz Schrank, Katerina Soulantika, Sergio Lentijo-Mozo, Beatriz Pelaz, Wolfgang Parak, Frank Ludwig, Jan Dieckhoff

Author Affiliations +

Proceedings Volume 9490, Advances in Global Health through Sensing Technologies 2015; 94900B (2015) https://doi.org/10.1117/12.2185642
Event: SPIE Sensing Technology + Applications, 2015, Baltimore, MD, United States

Abstract

Label-free optical schemes for molecular biosensing hold a strong promise for point-of-care applications in medical research and diagnostics. Apart from diagnostic requirements in terms of sensitivity, specificity, and multiplexing capability, also other aspects such as ease of use and manufacturability have to be considered in order to pave the way to a practical implementation. We present integrated optical waveguide as well as magnetic nanoparticle based molecular biosensor concepts that address these aspects. The integrated optical waveguide devices are based on low-loss photonic wires made of silicon nitride deposited by a CMOS compatible plasma-enhanced chemical vapor deposition (PECVD) process that allows for backend integration of waveguides on optoelectronic CMOS chips. The molecular detection principle relies on evanescent wave sensing in the 0.85 μm wavelength regime by means of Mach-Zehnder interferometers, which enables on-chip integration of silicon photodiodes and, thus, the realization of system-on-chip solutions. Our nanoparticle-based approach is based on optical observation of the dynamic response of functionalized magneticcore/ noble-metal-shell nanorods (‘nanoprobes’) to an externally applied time-varying magnetic field. As target molecules specifically bind to the surface of the nanoprobes, the observed dynamics of the nanoprobes changes, and the concentration of target molecules in the sample solution can be quantified. This approach is suitable for dynamic real-time measurements and only requires minimal sample preparation, thus presenting a highly promising point-of-care diagnostic system. In this paper, we present a prototype of a diagnostic device suitable for highly automated sample analysis by our nanoparticle-based approach.

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Joerg Schotter, Stefan Schrittwieser, Paul Muellner, Eva Melnik, Rainer Hainberger, Guenther Koppitsch, Franz Schrank, Katerina Soulantika, Sergio Lentijo-Mozo, Beatriz Pelaz, Wolfgang Parak, Frank Ludwig, and Jan Dieckhoff "Optical biosensor technologies for molecular diagnostics at the point-of-care", Proc. SPIE 9490, Advances in Global Health through Sensing Technologies 2015, 94900B (13 May 2015); https://doi.org/10.1117/12.2185642

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