Analyzing the texture changes in the quantitative phase maps of adipocytes

Darina Roitshtain; Orna Sharabani-Yosef; Amit Gefen; Natan T. Shaked

doi:10.1117/12.2209317

9 March 2016 Analyzing the texture changes in the quantitative phase maps of adipocytes

Darina Roitshtain, Orna Sharabani-Yosef, Amit Gefen, Natan T. Shaked

Proceedings Volume 9718, Quantitative Phase Imaging II; 97180R (2016) https://doi.org/10.1117/12.2209317
Event: SPIE BiOS, 2016, San Francisco, California, United States

Abstract

We present a new analysis tool for studying texture changes in the quantitative phase maps of live cells acquired by wide-field interferometry. The sensitivity of wide-field interferometry systems to small changes in refractive index enables visualizing cells and inner cell organelles without the using fluorescent dyes or other cell-invasive approaches, which may affect the measurement and require external labeling. Our label-free texture-analysis tool is based directly on the optical path delay profile of the sample and does not necessitate decoupling refractive index and thickness in the cell quantitative phase profile; thus, relevant parameters can be calculated using a single-frame acquisition. Our experimental system includes low-coherence wide-field interferometer, combined with simultaneous florescence microscopy system for validation. We used this system and analysis tool for studying lipid droplets formation in adipocytes. The latter demonstration is relevant for various cellular functions such as lipid metabolism, protein storage and degradation to viral replication. These processes are functionally linked to several physiological and pathological conditions, including obesity and metabolic diseases. Quantification of these biological phenomena based on the texture changes in the cell phase map has a potential as a new cellular diagnosis tool.

Conference Presentation

Citation Download Citation

Darina Roitshtain, Orna Sharabani-Yosef, Amit Gefen, and Natan T. Shaked "Analyzing the texture changes in the quantitative phase maps of adipocytes", Proc. SPIE 9718, Quantitative Phase Imaging II, 97180R (9 March 2016); https://doi.org/10.1117/12.2209317

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