Non-invasive high-contrast imaging of conjunctival goblet cells by moxifloxacin-based fluorescence microscopy

Ki Hean Kim; Seonghan Kim; Jungbin Lee; Young-Ho Jung; Chang Ho Yoon

doi:10.1117/12.2610724

7 March 2022 Non-invasive high-contrast imaging of conjunctival goblet cells by moxifloxacin-based fluorescence microscopy

Ki Hean Kim, Seonghan Kim, Jungbin Lee, Young-Ho Jung, Chang Ho Yoon

Author Affiliations +

Proceedings Volume PC11941, Ophthalmic Technologies XXXII; PC119410W (2022) https://doi.org/10.1117/12.2610724
Event: SPIE BiOS, 2022, San Francisco, California, United States

Abstract

Mucin secretive conjunctival goblet cells (CGCs) play important roles for ocular surface homeostasis by forming the mucous layer of tear film, so CGC examination is important for diagnosis of various ocular surface diseases. Here we show that CGCs can be non-invasively imaged in real time and in high contrasts in animal models by moxifloxacin-based fluorescence microscopy (MBFM) using moxifloxacin antibiotic ophthalmic solution for specific CGC labeling. Newly developed MBFM was applied to both disease mouse and rabbit models and it detected CGC damage and recovery via longitudinal imaging. These results showed that MBFM has potentials for non-invasive CGC examination.

Conference Presentation

Citation Download Citation

Ki Hean Kim, Seonghan Kim, Jungbin Lee, Young-Ho Jung, and Chang Ho Yoon "Non-invasive high-contrast imaging of conjunctival goblet cells by moxifloxacin-based fluorescence microscopy", Proc. SPIE PC11941, Ophthalmic Technologies XXXII, PC119410W (7 March 2022); https://doi.org/10.1117/12.2610724

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