CONFERENCE PROCEEDINGS
Advanced Search
Home > Proceedings > Volume PC11966 > Article
Presentation
2 March 2022 Three-dimensional Bi-functional Refractive Index and Fluorescence microscopy (BRIEF)
Yi Xue, David Ren, Laura Waller
Author Affiliations +
Proceedings Volume PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX; PC1196608 (2022) https://doi.org/10.1117/12.2610559
Event: SPIE BiOS, 2022, San Francisco, California, United States
Abstract
Conventional microscopy usually has a single function aiming to measure one type of signal, such as fluorescence microscopy or phase microscopy. Here, we develop a new computational hybrid imaging method, based on a multi-slice multiple scattering model, that reconstructs both 3D fluorescence and 3D RI by solving an inverse problem from a single dataset of dozens fluorescence images captured at a single image plane. This multi-functional system not only bridges the gap between fluorescence and RI imaging, but also can digitally correct multiple scattering effects in the fluorescence images by using the phantom structure recovered by the reconstructed 3D RI.
Conference Presentation
© (2022) COPYRIGHT Society of Photo-Optical Instrumentation Engineers (SPIE). Downloading of the abstract is permitted for personal use only.
Citation Download Citation
Yi Xue, David Ren, and Laura Waller "Three-dimensional Bi-functional Refractive Index and Fluorescence microscopy (BRIEF)", Proc. SPIE PC11966, Three-Dimensional and Multidimensional Microscopy: Image Acquisition and Processing XXIX, PC1196608 (2 March 2022); https://doi.org/10.1117/12.2610559
ACCESS THE FULL ARTICLE
ORGANIZATIONAL
Sign in with credentials provided by your organization.
INSTITUTIONAL
Select your institution to access the SPIE Digital Library.
SELECT YOUR INSTITUTION
PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.
PERSONAL SIGN IN
No SPIE Account? Create one
PURCHASE THIS CONTENT
SUBSCRIBE TO DIGITAL LIBRARY
50 downloads per 1-year subscription
Members: $195
Non-members: $335 ADD TO CART
25 downloads per 1 - year subscription
Members: $145
Non-members: $250 ADD TO CART
PURCHASE SINGLE ARTICLE
Includes PDF, HTML & Video, when available
Members: $17.00
Non-members: $21.00 ADD TO CART
PROCEEDINGS
 PRESENTATION
WATCH
PRESENTATION SAVE TO MY LIBRARY
GET CITATION
Advertisement
Advertisement
KEYWORDS
Luminescence
3D image reconstruction
Microscopy
Refractive index
3D image processing
3D modeling
Image quality
RELATED CONTENT
Subscribe to Digital Library
Receive Erratum Email Alert
Back to Top