Detection of Legionella pneumophila with antimicrobial peptide-based GaAs/AlGaAs biosensor

M. Amirul Islam; Walid M. Hassen; Azam F. Tayabali; Jan J. Dubowski

doi:10.1117/12.2626470

27 March 2022 Detection of Legionella pneumophila with antimicrobial peptide-based GaAs/AlGaAs biosensor

M. Amirul Islam, Walid M. Hassen, Azam F. Tayabali, Jan J. Dubowski

Proceedings Volume PC11990, Nanoscale and Quantum Materials: From Synthesis and Laser Processing to Applications 2022; PC119900C (2022) https://doi.org/10.1117/12.2626470
Event: SPIE LASE, 2022, San Francisco, California, United States

Abstract

Biosensor-based detection of pathogenic bacteria has gained attention since it could be fast, portable, cost effective and potentially easy to use. In this study, we investigated the detection of L. pneumophila using an antimicrobial peptide (AMP) and antibody (Ab) functionalized GaAs/AlGaAs biochips. The AMP attachment on GaAs surface was evaluated using Fourier-transform infrared spectroscopy (FTIR) and atomic force microscopy (AFM). The peptide-related absorbance bands in IR (1588 cm^-1, 1653 cm^-1, and 1734 cm^-1) suggest the successful immobilization of AMP on GaAs. The bacterial capture efficiency/affinity on GaAs surface was evaluated for several peptides such as warnericin RK, clavanin, parasin, magainin, melittin and it was observed that the warnericin RK obtained ~4 times higher capture efficiency compared to the other peptides. We successfully detected L. pneumophila using AMP, as well as Ab conjugated GaAs/AlGaAs biosensors. The AMP functionalized biosensors, however, allowed higher sensitivity compared to Ab based bioarchitecture. The proposed AMP functionalized GaAs/AlGaAs biosensor is attractive for rapid and sensitive detection of L. pneumophila in water samples.

Conference Presentation

Citation Download Citation

M. Amirul Islam, Walid M. Hassen, Azam F. Tayabali, and Jan J. Dubowski "Detection of Legionella pneumophila with antimicrobial peptide-based GaAs/AlGaAs biosensor", Proc. SPIE PC11990, Nanoscale and Quantum Materials: From Synthesis and Laser Processing to Applications 2022, PC119900C (27 March 2022); https://doi.org/10.1117/12.2626470

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available