Understanding neurodegeneration in human organoid retina with optical microscopy

Jürgen W. Czarske; Nektarios Koukourakis; Stefan Rothe; Felix Wagner; Mike Karl

doi:10.1117/12.2622155

30 May 2022 Understanding neurodegeneration in human organoid retina with optical microscopy

Jürgen W. Czarske, Nektarios Koukourakis, Stefan Rothe, Felix Wagner, Mike Karl

Proceedings Volume PC12144, Biomedical Spectroscopy, Microscopy, and Imaging II; PC121440M (2022) https://doi.org/10.1117/12.2622155
Event: SPIE Photonics Europe, 2022, Strasbourg, France

Abstract

The retina is an epithelium composed of different cell layers with unique optical properties and detects light by photoreceptor neurons for visual function. The quest for suitable measurement methods to detect the health status of retinal tissues is ongoing. We study the capability of the optical transmission matrix, which fully describes the transition of a light field propagating through a scattering sample. Despite its rich information content, the transmission matrix is commonly just used for light delivery through scattering media. Digital holography is employed to measure the transmitted light. We demonstrate that singular value decomposition of the transmission matrix allows to discriminate phantom tissues with varying scattering coefficient. We apply these findings to retinal organoid tissues. Application of an inducer of retinal damage in animals, caused cell death and structural changes in human retinal organoids, which resulted in distinct changes in the transmission matrix. Our data indicate that the analysis of the transmis-sion matrix can distinguish pathologic changes of the retina towards the development of imag-ing-based biomarkers. Laser microscopy of retinal organoid samples from human induced plu-ripotent stem cells is a disruptive technology that promises paradigm shifts for biomedicine.

Conference Presentation

Citation Download Citation

Jürgen W. Czarske, Nektarios Koukourakis, Stefan Rothe, Felix Wagner, and Mike Karl "Understanding neurodegeneration in human organoid retina with optical microscopy", Proc. SPIE PC12144, Biomedical Spectroscopy, Microscopy, and Imaging II, PC121440M (30 May 2022); https://doi.org/10.1117/12.2622155

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available

Members: $17.00

Non-members: $21.00 ADD TO CART

PROCEEDINGS
PRESENTATION

WATCH
PRESENTATION SAVE TO MY LIBRARY

GET CITATION

KEYWORDS

Tissues

Retina

Light scattering

Neurodegeneration

Optical microscopy

Cell death

Scattering

Show All Keywords

Keywords/Phrases

Search In:

Publication Years