Label-free clinical metabolic imaging of epithelial tissue with multi-wavelength excitation and multispectral emission autofluorescence lifetime endoscopy

Javier A. Jo; Michael J. Serafino; Gabriel P. Tortorelli; Ronald C. Faram; Kathleen E. Higgins; Sharukh S. Khajotia; Yi-Shing Lisa Cheng D.D.S.; Jacqueline M. Plemons; Victoria Woo; Kayla R. Caughlin

doi:10.1117/12.2648480

17 March 2023 Label-free clinical metabolic imaging of epithelial tissue with multi-wavelength excitation and multispectral emission autofluorescence lifetime endoscopy

Javier A. Jo, Michael J. Serafino, Gabriel P. Tortorelli, Ronald C. Faram, Kathleen E. Higgins, Sharukh S. Khajotia, Yi-Shing Lisa Cheng D.D.S., Jacqueline M. Plemons, Victoria Woo, Kayla R. Caughlin

Author Affiliations +

Proceedings Volume PC12363, Multiscale Imaging and Spectroscopy IV; PC123630H (2023) https://doi.org/10.1117/12.2648480
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

We have recently developed a novel, cost-effective, versatile, and practical frequency-domain (FD) FLIM implementation capable of simultaneous multi-wavelength excitation, simultaneous multispectral detection, and sub-nanosecond to nanosecond fluorescence lifetime estimation. This novel technology has been adopted to implement an FD-FLIM endoscopy system for label-free metabolic imaging of epithelial tissues. The FD-FLIM endoscopy system is capable of simultaneous excitation at two wavelengths (375-nm for NADH, 445-nm for FAD). Preliminary results indicate that established metabolic autofluorescence biomarkers of epithelial pre-malignancy and malignancy can be clinically imaged with this novel FD-FLIM endoscopy technology. Future work will focus on exploring clinical applications of label-free metabolic imaging using this novel FD-FLIM endoscopy technology.

Conference Presentation

Citation Download Citation

Javier A. Jo, Michael J. Serafino, Gabriel P. Tortorelli, Ronald C. Faram, Kathleen E. Higgins, Sharukh S. Khajotia, Yi-Shing Lisa Cheng D.D.S., Jacqueline M. Plemons, Victoria Woo, and Kayla R. Caughlin "Label-free clinical metabolic imaging of epithelial tissue with multi-wavelength excitation and multispectral emission autofluorescence lifetime endoscopy", Proc. SPIE PC12363, Multiscale Imaging and Spectroscopy IV, PC123630H (17 March 2023); https://doi.org/10.1117/12.2648480

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