Dr. Michael J. Serafino Profile

Dr. Michael J. Serafino

at The Univ. of Southern California

SPIE Involvement:

Author

Publications (17)

Proceedings Article | 13 March 2024 Poster + Paper

Enhancing roll-off performance in SD-OCT with a frequency comb

Regina Magalhaes, Michael Serafino, John Oghalai, Brian Applegate

Proceedings Volume 12830, 128300K (2024) https://doi.org/10.1117/12.3005759

KEYWORDS: Frequency combs, Optical coherence tomography, Signal detection, Imaging systems, Ear, Tunable filters, Design, Light sources, Optical isolators, Spectrometers

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Proceedings Article | 13 March 2024 Presentation + Paper

Hand-held optical coherence tomography endoscope and stitching algorithm for imaging the human cochlea via the round window

Julianna Bordas, Wihan Kim, Michael Serafino, Jack Tang, Dorothy Pan, John Oghalai, Brian Applegate

Proceedings Volume 12830, 128300O (2024) https://doi.org/10.1117/12.3005704

KEYWORDS: Endoscopes, Windows, Ear, Optical coherence tomography, Cochlea, Diseases and disorders, Biological imaging, Detection and tracking algorithms, Algorithm development, X-ray computed tomography

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Proceedings Article | 13 March 2024 Presentation + Paper

Dynamic optical coherence tomography strongly enhances contrast in the ex vivo murine cochlea

Michael Serafino, Clayton Walker, Patricia Quiñones, Juemei Wang, Frank Macías-Escrivá, John Oghalai, Brian Applegate

Proceedings Volume 12830, 128300X (2024) https://doi.org/10.1117/12.3005635

KEYWORDS: Optical coherence tomography, Cochlea, Interferograms, RGB color model, Image quality standards, Anatomy, Visualization, Nerve, Neodymium, In vivo imaging

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Proceedings Article | 17 March 2023 Presentation

Label-free clinical metabolic imaging of epithelial tissue with multi-wavelength excitation and multispectral emission autofluorescence lifetime endoscopy

Javier Jo, Michael Serafino, Gabriel Tortorelli, Ronald Faram, Kathleen Higgins, Sharukh Khajotia, Yi-Shing Cheng, Jacqueline Plemons, Victoria Woo, Kayla Caughlin

Proceedings Volume PC12363, PC123630H (2023) https://doi.org/10.1117/12.2648480

KEYWORDS: Endoscopy, Tissues, Luminescence, Imaging systems, Multispectral imaging, Image resolution, Fluorescence spectroscopy, Fluorescence lifetime imaging

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Proceedings Article | 17 March 2023 Presentation

Dual-excitation multispectral autofluorescence lifetime endoscopy for clinical label-free metabolic imaging of oral lesions

Rodrigo Cuenca Martinez, Michael Serafino, Gabriel Tortorelli, Ronald Faram, Kathleen Higgins, Sharukh Khajotia, Yi-Shing Cheng, Jacqueline Plemons, Victoria Woo, Carlos Busso Recabarren, Kayla Caughlin, Javier Jo

Proceedings Volume 12354, 1235409 (2023) https://doi.org/10.1117/12.2647972

KEYWORDS: Endoscopy, Imaging systems, Cancer, Tissues, Multispectral imaging, Luminescence, Image resolution, Fluorescence spectroscopy, Fluorescence lifetime imaging

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Proceedings Article | 16 March 2023 Presentation

Practical and cost-effective implementations of fluorescence lifetime imaging systems (Conference Presentation)

Michael Serafino, Javier Jo

Proceedings Volume PC12391, PC1239103 (2023) https://doi.org/10.1117/12.2647982

KEYWORDS: Luminescence, Fluorescence lifetime imaging, Imaging systems, Modulation, Field programmable gate arrays, Time resolved spectroscopy, Semiconductor lasers, Pulsed laser operation, Photodetectors, Phase shift keying

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Proceedings Article | 7 March 2022 Presentation

Label-free clinical metabolic and biochemical imaging of the oral epithelial cancer enabled by autofluorescence lifetime imaging endoscopy

Rodrigo Cuenca Martinez, Michael Serafino, Gabriel Tortorelli, Sharukh Khajotia, Ronald Faram, David Lewis, Lida Radfar, Kathleen Higgins, Yi-Shing Cheng, Javier Jo

Proceedings Volume PC11935, PC1193509 (2022) https://doi.org/10.1117/12.2609017

KEYWORDS: Endoscopy, Cancer, Multispectral imaging, Luminescence, Tissues, Tissue optics, Time resolved spectroscopy, Statistical analysis, Proteins, Medicine

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Proceedings Article | 7 March 2022 Presentation

Dual-wavelength excitation multispectral autofluorescence lifetime imaging handheld probe for label-free metabolic clinical imaging of the oral mucosa

Gabriel Tortorelli, Rodrigo Cuenca Martinez, Michael Serafino, Wihan Kim, Brian Applegate, Javier Jo

Proceedings Volume PC11935, PC1193508 (2022) https://doi.org/10.1117/12.2608936

KEYWORDS: Multispectral imaging, Mirrors, Lenses, Tissues, Time resolved spectroscopy, Relays, Objectives, Medicine, Luminescence, Human subjects

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Proceedings Article | 2 March 2022 Presentation

Versatile and cost-effective multi-wavelength excitation and multispectral-emission frequency-domain fluorescence lifetime imaging system for real-time biomedical and clinical applications

Michael Serafino, Javier Jo

Proceedings Volume PC11972, PC119720F (2022) https://doi.org/10.1117/12.2608927

KEYWORDS: Imaging systems, Fluorescence lifetime imaging, Field programmable gate arrays, Biomedical optics, Software development, Modulation, Phase shift keying, Signal to noise ratio, Semiconductor lasers, Luminescence

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SPIE Journal Paper | 30 September 2020 Open Access

Dual-modality optical coherence tomography and frequency-domain fluorescence lifetime imaging microscope system for intravascular imaging

Xi Chen, Wihan Kim, Michael Serafino, Zheng Tan, Javier Jo, Brian Applegate

JBO, Vol. 25, Issue 09, 096010, (September 2020) https://doi.org/10.1117/12.10.1117/1.JBO.25.9.096010

KEYWORDS: Optical coherence tomography, Endoscopes, Fluorescence lifetime imaging, Luminescence, Cladding, Imaging systems, Arteries, Absorption, Silica, Polishing

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Significance: Detailed biochemical and morphological imaging of the plaque burdened coronary arteries holds the promise of improved understanding of atherosclerosis plaque development, ultimately leading to better diagnostics and therapies. Aim: Development of a dual-modality intravascular catheter supporting swept-source optical coherence tomography (OCT) and frequency-domain fluorescence lifetime imaging (FD-FLIM) of endogenous fluorophores with UV excitation. Approach: We instituted a refined approach to endoscope development that combines simulation in a commercial ray tracing program, fabrication, and a measurement method for optimizing ball-lens performance. With this approach, we designed and developed a dual-modality catheter endoscope based on a double-clad fiber supporting OCT through the core and fluorescence collection through the first cladding. We varied the relative percent of UV excitation launched into the core and first cladding to explore the potential resolution improvement for FD-FLIM. The developed catheter endoscope was optically characterized, including measurement of spatial resolution and fluorescent lifetimes of standard fluorophores. Finally, the system was demonstrated on fresh ex vivo human coronary arteries. Results: The developed endoscope was shown to have optical performance similar to predictions derived from the simulation approach. The FLIM resolution can be improved by over a factor of 4 by primarily illuminating through the core rather than the first cladding. However, time-dependent solarization losses need to be considered when choosing the relative percentage. We ultimately chose to illuminate with 7% of the power transmitting through the core. The resulting catheter endoscope had 40-μm lateral resolution for OCT and <100 μm lateral resolution for FD-FLIM. Images of ex vivo coronary arteries are consistent with expectations based on histopathology. Conclusions: The results demonstrate that our approach for endoscope simulation produces reliable predictions of endoscope performance. Simulation results guided our development of a multimodal OCT/FD-FLIM catheter imaging system for investigating atherosclerosis in coronary arteries.

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Proceedings Article | 7 March 2019 Presentation

A compact and robust handheld frequency-domain fluorescence lifetime imaging (FLIM) endoscope for clinical label-free metabolic imaging of oral cancer (Conference Presentation)

Oscar Benavides, Michael Serafino, Shuna Cheng, Javier Jo

Proceedings Volume 10853, 108530M (2019) https://doi.org/10.1117/12.2524140

KEYWORDS: Fluorescence lifetime imaging, Endoscopes, Cancer, Imaging systems, Luminescence, Endoscopy, Microelectromechanical systems, Scanners, Mirrors, Continuous wave operation

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Proceedings Article | 14 March 2018 Presentation

Compact and cost-effective handheld multispectral fluorescence lifetime imaging (FLIM) system for oral mucosa in vivo imaging based on a frequency-domain implementation (Conference Presentation)

Shuna Cheng, Michael Serafino, Rodrigo Cuenca, Beena Ahmed, Brian Applegate, Javier Jo

Proceedings Volume 10469, 104690O (2018) https://doi.org/10.1117/12.2287855

KEYWORDS: Fluorescence lifetime imaging, Imaging systems, In vivo imaging, Lenses, Relays, Signal processing, Multispectral imaging, Endoscopes, Microelectromechanical systems, Scanners

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Proceedings Article | 14 March 2018 Presentation

Automatic detection of macrophages/foam cells in coronary atherosclerotic plaques based on fluorescence lifetime imaging (FLIM) (Conference Presentation)

Jose Rico-Jimenez, Michael Serafino, Xi Chen, Sebina Shrestha, Wihan Kim, Brian Walton, Brian Applegate, Javier Jo

Proceedings Volume 10471, 1047105 (2018) https://doi.org/10.1117/12.2288606

KEYWORDS: Fluorescence lifetime imaging, Multispectral imaging, Foam, Microscopy, Image analysis, Machine learning, Tissues, Receivers, Statistical analysis

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Proceedings Article | 14 March 2018 Presentation

Design and validation of the ball lens-based intravascular catheter for fluorescence lifetime imaging microscopy of atherosclerosis (Conference Presentation)

Xi Chen, Wihan Kim, Michael Serafino, Zheng Tan, Brian Walton, L. Maximilian Buja, Jessie Adame, Javier Jo, Brian Applegate

Proceedings Volume 10471, 1047102 (2018) https://doi.org/10.1117/12.2287447

KEYWORDS: Fluorescence lifetime imaging, Microscopy, Endoscopes, Luminescence, Endoscopy, Tissues, Fiber optics, Performance modeling, Zemax, Polishing

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Proceedings Article | 25 January 2018 Presentation

Design and validation of the ball lens-based intravascular catheter for fluorescence lifetime imaging microscopy of atherosclerosis (Conference Presentation)

Xi Chen, Wihan Kim, Michael Serafino, Javier Jo, Brian Applegate

Proceedings Volume 9689, 968932 (2018) https://doi.org/10.1117/12.2211764

Proceedings Article | 1 May 2017 Presentation

Toward intravascular morphological and biochemical imaging of atherosclerosis with optical coherence tomography (OCT) and fluorescence lifetime imaging (FLIM) (Conference Presentation)

Xi Chen, Wihan Kim, Michael Serafino, Brian Walton, Javier Jo, Brian Applegate

Proceedings Volume 10060, 1006007 (2017) https://doi.org/10.1117/12.2256676

KEYWORDS: Shortwaves, Optical coherence tomography, Fluorescence lifetime imaging, Imaging systems, Endoscopes, Infrared imaging, Lens design, Arteries, Therapeutics, Near ultraviolet

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SPIE Journal Paper | 27 August 2014 Open Access

Automated classification of optical coherence tomography images for the diagnosis of oral malignancy in the hamster cheek pouch

Paritosh Pande, Sebina Shrestha, Jesung Park, Michael Serafino, Irma Gimenez-Conti, Jimi Brandon, Yi-Shing Cheng, Brian Applegate, Javier Jo

JBO, Vol. 19, Issue 08, 086022, (August 2014) https://doi.org/10.1117/12.10.1117/1.JBO.19.8.086022

KEYWORDS: Optical coherence tomography, Tissues, Image classification, Image segmentation, Cancer, Feature selection, Image processing, Image analysis, Tumor growth modeling, Imaging systems

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