Dynamic optical coherence tomography strongly enhances contrast in the ex vivo murine cochlea

Michael J. Serafino; Clayton Walker; Patricia M. Quiñones; Juemei Wang; Frank D. Macías-Escrivá; John S. Oghalai; Brian E. Applegate

doi:10.1117/12.3005635

12 March 2024 Dynamic optical coherence tomography strongly enhances contrast in the ex vivo murine cochlea

Michael J. Serafino, Clayton Walker, Patricia M. Quiñones, Juemei Wang, Frank D. Macías-Escrivá, John S. Oghalai, Brian E. Applegate

Author Affiliations +

Proceedings Volume 12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII; 128300X (2024) https://doi.org/10.1117/12.3005635
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

We report dynamic optical coherence tomography (D-OCT) images of the organ of Corti (ooC) in ex vivo mouse cochleas. The ooC is responsible for transducing sound-evoked mechanical vibrations and amplifying them in the process of hearing. Thorough knowledge of the micromechanical properties of the ooC is required for understanding how hearing functions. Recently, OCT has emerged as a safe and effective tool to probe the inner workings of the cochlea and ooC. However, OCT is limited in its ability to directly resolve cellular architecture due to limited optical scattering-based contrast between different cell types. D-OCT is a label-free method capable of probing sub-resolution movements by analyzing speckle and phase information from standard OCT data as a function of time. We show that key structures in the ooC can be identified with D-OCT versus standard OCT, and that D-OCT has the potential to characterize the ooC and advance our understanding of the process of hearing.

Conference Presentation

(2024) Published by SPIE. Downloading of the abstract is permitted for personal use only.

Citation Download Citation

Michael J. Serafino, Clayton Walker, Patricia M. Quiñones, Juemei Wang, Frank D. Macías-Escrivá, John S. Oghalai, and Brian E. Applegate "Dynamic optical coherence tomography strongly enhances contrast in the ex vivo murine cochlea", Proc. SPIE 12830, Optical Coherence Tomography and Coherence Domain Optical Methods in Biomedicine XXVIII, 128300X (12 March 2024); https://doi.org/10.1117/12.3005635

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