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6 March 2023 Oblique back illumination cell phone microscopy for blood analysis
Marisa M. Morakis, Gregory N. McKay, Eli J. Foster, Julie L. Bentley, Nicholas J. Durr
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Proceedings Volume PC12369, Optics and Biophotonics in Low-Resource Settings IX; PC123690B (2023) https://doi.org/10.1117/12.2650918
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
The complete blood count (CBC) is a foundational diagnostic test, but its accessibility is limited due to the blood draw, expensive laboratory equipment, and trained personnel required. Here, we present a cell phone microscope design for achieving phase contrast in high resolution capillary imaging, which allows individual blood cells to be imaged for a non-invasive CBC. The cell phone microscope uses a reversed lens as an objective to maintain a high resolution. Relay lenses create space for incorporation of an offset LED that can be critically imaged to produce oblique back illumination, resulting in phase contrast.
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Marisa M. Morakis, Gregory N. McKay, Eli J. Foster, Julie L. Bentley, and Nicholas J. Durr "Oblique back illumination cell phone microscopy for blood analysis", Proc. SPIE PC12369, Optics and Biophotonics in Low-Resource Settings IX, PC123690B (6 March 2023); https://doi.org/10.1117/12.2650918
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KEYWORDS
Blood
Cell phones
Microscopy
Point-of-care devices
Phase contrast
Microscopes
Relays
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