High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)

Kayvan F. Tehrani; Alejandro De la Cadena; Stephen A. Boppart

doi:10.1117/12.2648069

16 March 2023 High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)

Kayvan F. Tehrani, Alejandro De la Cadena, Stephen A. Boppart

Author Affiliations +

Proceedings Volume PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII; PC1239009 (2023) https://doi.org/10.1117/12.2648069
Event: SPIE BiOS, 2023, San Francisco, California, United States

Abstract

Nonlinear label-free microscopy techniques have shown great promise for analyzing biological tissues with an unmatched level of information. These techniques are capable of identifying intrinsic optical signals generated in response to ultrafast laser pulses. A roadblock in fast label-free imaging is the limited signal-to-noise ratio (SNR), due to the low number of photons close to the noise level. We implemented heterodyne detection of the third-order signals to overcome this limit and surpass the 1/f noise. Our method increases the SNR by a factor of 2.3, leading to a factor of 5 reduction in imaging time.

Conference Presentation

Citation Download Citation

Kayvan F. Tehrani, Alejandro De la Cadena, and Stephen A. Boppart "High signal-to-noise ratio rapid third-order label-free and harmonic generation microscopy of neural dynamics through electronic heterodyne amplification (Conference Presentation)", Proc. SPIE PC12390, High-Speed Biomedical Imaging and Spectroscopy VIII, PC1239009 (16 March 2023); https://doi.org/10.1117/12.2648069

ACCESS THE FULL ARTICLE

INSTITUTIONAL
Select your institution to access the SPIE Digital Library.

SELECT YOUR INSTITUTION

PERSONAL
Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.

PERSONAL SIGN IN

No SPIE Account? Create one

PURCHASE THIS CONTENT

SUBSCRIBE TO DIGITAL LIBRARY

50 downloads per 1-year subscription

Members: $195

Non-members: $335 ADD TO CART

25 downloads per 1 - year subscription

Members: $145

Non-members: $250 ADD TO CART

PURCHASE SINGLE ARTICLE

Includes PDF, HTML & Video, when available