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15 March 2023 Super-resolution stimulated Raman scattering of opaque tissues using speckle patterns (Conference Presentation)
Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, Hilton Barbosa de Aguiar
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Proceedings Volume PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023; PC123920J (2023) https://doi.org/10.1117/12.2649896
Event: SPIE BiOS, 2023, San Francisco, California, United States
Abstract
We demonstrate a novel approach to super-resolution in stimulated Raman scattering based on single-pixel detection scheme. We exploit speckle structured illumination, and enable fast super-resolution microscopy using Raman contrasts. The methodology is straightforward to implement and provides label-free super-resolution imaging of optically thick specimens, therefore paving the way for probing complex biological systems when exogenous labelling is challenging.
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Julien Guilbert, Awoke Negash, Simon Labouesse, Sylvain Gigan, Anne Sentenac, and Hilton Barbosa de Aguiar "Super-resolution stimulated Raman scattering of opaque tissues using speckle patterns (Conference Presentation)", Proc. SPIE PC12392, Advanced Chemical Microscopy for Life Science and Translational Medicine 2023, PC123920J (15 March 2023); https://doi.org/10.1117/12.2649896
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KEYWORDS
Super resolution
Tissues
Opacity
Raman scattering
Microscopy
Speckle pattern
Imaging systems
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