Raman spectroscopic immune cell phenotyping: macrophages and intra-epithelial lymphocytes

Max Naumann; Rustam Guliev; Simone Eiserloh; Martin Dinkel; Natalie Arend; Ignacio Rubio; Oliver Werz; Iwan W. Schie; Kai Hildner; Michael Bauer; Jürgen Popp; Ute Neugebauer

doi:10.1117/12.3005343

13 March 2024 Raman spectroscopic immune cell phenotyping: macrophages and intra-epithelial lymphocytes

Max Naumann, Rustam Guliev, Simone Eiserloh, Martin Dinkel, Natalie Arend, Ignacio Rubio, Oliver Werz, Iwan W. Schie, Kai Hildner, Michael Bauer, Jürgen Popp, Ute Neugebauer

Author Affiliations +

Proceedings Volume PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024; PC1282201 (2024) https://doi.org/10.1117/12.3005343
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Raman spectroscopy can be utilized for advanced immunological characterization of different immune cells. Macrophages, important immune cells found in almost all tissues, develop different functional phenotypes. Raman spectroscopic fingerprints of cells point to differences in lipid composition and allowed a reliable differentiation of the different phenotypes using statistical models. Raman analysis of mixed cell populations isolated from the small intestine intraepithelial lymphocyte compartment from a mouse model of T cell-deficient mice with and without T cell transfer revealed two major subpopulations: one was made up of larger cells (e.g., epithelial cells or myeloid cells/macrophages), and one revealed typical spectral features of T cells. Quantification of the latter from the Raman data was in excellent agreement with flow cytometry data.

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Max Naumann, Rustam Guliev, Simone Eiserloh, Martin Dinkel, Natalie Arend, Ignacio Rubio, Oliver Werz, Iwan W. Schie, Kai Hildner, Michael Bauer, Jürgen Popp, and Ute Neugebauer "Raman spectroscopic immune cell phenotyping: macrophages and intra-epithelial lymphocytes", Proc. SPIE PC12822, Photonic Diagnosis, Monitoring, Prevention, and Treatment of Infections and Inflammatory Diseases 2024, PC1282201 (13 March 2024); https://doi.org/10.1117/12.3005343

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KEYWORDS

Raman spectroscopy

Cell phenotyping

Spectroscopy

Flow cytometry

Imaging spectroscopy

In vitro testing

Photonics

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