Raman spectroscopy can be utilized for advanced immunological characterization of different immune cells. Macrophages, important immune cells found in almost all tissues, develop different functional phenotypes. Raman spectroscopic fingerprints of cells point to differences in lipid composition and allowed a reliable differentiation of the different phenotypes using statistical models. Raman analysis of mixed cell populations isolated from the small intestine intraepithelial lymphocyte compartment from a mouse model of T cell-deficient mice with and without T cell transfer revealed two major subpopulations: one was made up of larger cells (e.g., epithelial cells or myeloid cells/macrophages), and one revealed typical spectral features of T cells. Quantification of the latter from the Raman data was in excellent agreement with flow cytometry data.
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