Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

Yayao Ma; Luzhe Huang; Chandani Sen; Samuel Burri; Claudio Bruschini; Xilin Yang; Robert Cameron; Gregory Fishbein; Brigitte Gomperts; Aydogan Ozcan; Edoardo Charbon; Liang Gao

doi:10.1117/12.3002375

13 March 2024 Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)

Yayao Ma, Luzhe Huang, Chandani Sen, Samuel Burri, Claudio Bruschini, Xilin Yang, Robert Cameron, Gregory Fishbein, Brigitte Gomperts, Aydogan Ozcan, Edoardo Charbon, Liang Gao

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Proceedings Volume PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences; PC1285712 (2024) https://doi.org/10.1117/12.3002375
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence lifetime imaging microscopy (FLIM) measures fluorescence lifetimes of fluorescent probes to investigate molecular interactions. However, conventional FLIM systems often requires extensive scanning that is time-consuming. To address this challenge, we developed a novel computational imaging technique called light field tomographic FLIM (LIFT-FLIM). Our approach acquires volumetric fluorescence lifetime images in a highly data-efficient manner, significantly reducing the number of scanning steps. We demonstrated LIFT-FLIM using a single-photon avalanche diode array on various biological systems. Additionally, we expanded to spectral FLIM and demonstrated high-content multiplexed imaging of lung organoids. LIFT-FLIM can open new avenues in the biomedical research.

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Yayao Ma, Luzhe Huang, Chandani Sen, Samuel Burri, Claudio Bruschini, Xilin Yang, Robert Cameron, Gregory Fishbein, Brigitte Gomperts, Aydogan Ozcan, Edoardo Charbon, and Liang Gao "Light-field tomographic fluorescence lifetime imaging microscopy (LIFT-FLIM)", Proc. SPIE PC12857, Computational Optical Imaging and Artificial Intelligence in Biomedical Sciences, PC1285712 (13 March 2024); https://doi.org/10.1117/12.3002375

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KEYWORDS

Fluorescence lifetime imaging

Tomography

Single photon avalanche diodes

Time lapse microscopy

Imaging arrays

3D image processing

Biomedical optics

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