Low quantum yield fluorophores: an obstacle or an opportunity?

Vladislav V. Yakovlev; Vsevolod Cheburkanov; Jason W. Pipal

doi:10.1117/12.3006260

13 March 2024 Low quantum yield fluorophores: an obstacle or an opportunity?

Vladislav V. Yakovlev, Vsevolod Cheburkanov, Jason W. Pipal

Proceedings Volume PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV; PC1286208 (2024) https://doi.org/10.1117/12.3006260
Event: SPIE BiOS, 2024, San Francisco, California, United States

Abstract

Fluorescence microscopy relies on efficient emission of molecules excited by an incident laser light. However, their emission is often intrinsically limited by a low fluorescence quantum yield which results in low contrast images. In our recent efforts, we explored the fluorescence life-time imaging contrast for those low efficient fluorophores to find out that those molecules exhibit a rather intense emission over a relatively short time. This short fluorescence time was found to be dependent of the local structure and local environment providing a novel biomarker for biological imaging.

Conference Presentation

Citation Download Citation

Vladislav V. Yakovlev, Vsevolod Cheburkanov, and Jason W. Pipal "Low quantum yield fluorophores: an obstacle or an opportunity?", Proc. SPIE PC12862, Reporters, Markers, Dyes, Nanoparticles, and Molecular Probes for Biomedical Applications XV, PC1286208 (13 March 2024); https://doi.org/10.1117/12.3006260

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