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1 May 2010 Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy
Wen Lo, Tsung-Jen Wang, Wei-Liang Chen, Chiu-Mei Hsueh, Shean-Jen Chen, Yang-Fang Chen, Hsiu-Chu Chou, Pi-Jung Lin M.D., Fung-Rong Hu, Chen-Yuan Dong
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Journal of Biomedical Optics, Vol. 15, Issue 3, 036005 (May 2010). https://doi.org/10.1117/1.3432718
Abstract
We applied multiphoton autofluorescence (MAF) and second-harmonic generation (SHG) microscopy to monitor corneal wound healing after photorefractive keratectomy (PRK). Our results show that keratocyte activation can be observed by an increase in its MAF, while SHG imaging of corneal stroma can show the depletion of Bowman's layer after PRK and the reticular collagen deposition in the wound healing stage. Furthermore, quantification of the keratocyte activation and collagen deposition in conjunction with immunohistochemistry and histological images demonstrate the effectiveness of mitomycin C (MMC) in suppressing myofibroblast proliferation and collagen regeneration in the post-PRK wound healing process.
©(2010) Society of Photo-Optical Instrumentation Engineers (SPIE)
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Wen Lo, Tsung-Jen Wang, Wei-Liang Chen, Chiu-Mei Hsueh, Shean-Jen Chen, Yang-Fang Chen, Hsiu-Chu Chou, Pi-Jung Lin M.D., Fung-Rong Hu, and Chen-Yuan Dong "Label-free structural characterization of mitomycin C-modulated wound healing after photorefractive keratectomy by the use of multiphoton microscopy," Journal of Biomedical Optics 15(3), 036005 (1 May 2010). https://doi.org/10.1117/1.3432718
Published: 1 May 2010
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KEYWORDS
Second-harmonic generation
Cornea
Collagen
Wound healing
Multiphoton microscopy
Ophthalmology
Physics
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