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11 March 2015 Noninvasive photoacoustic microscopy of methemoglobin in vivo
Min Tang, Yong Zhou, Ruiying Zhang, Lihong V. Wang
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Journal of Biomedical Optics, Vol. 20, Issue 3, 036007 (March 2015). https://doi.org/10.1117/1.JBO.20.3.036007
Abstract
Due to the various causes of methemoglobinemia and its potential to be confused with other diseases, in vivo measurements of methemoglobin have significant applications in the clinic. Using photoacoustic microscopy (PAM), we quantified the average and the distributed percentage of methemoglobin both in vitro and in vivo. Based on the absorption spectra of methemoglobin, oxyhemoglobin, and deoxyhemoglobin, three wavelengths were chosen to differentiate methemoglobin from the others. The methemoglobin concentrations calculated from the photoacoustic signals agreed well with the preset concentrations. Then we imaged the methemoglobin percentage in microtubes that mimicked blood vessels. Average percentages calculated for five samples with different methemoglobin concentrations also agreed well with the preset values. Finally, we demonstrated the ability of PAM to detect methemoglobin in vivo in a mouse ear. Our results show that PAM can quantitatively image methemoglobin distribution in vivo.
© 2015 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2015/$25.00 © 2015 SPIE
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Min Tang, Yong Zhou, Ruiying Zhang, and Lihong V. Wang "Noninvasive photoacoustic microscopy of methemoglobin in vivo," Journal of Biomedical Optics 20(3), 036007 (11 March 2015). https://doi.org/10.1117/1.JBO.20.3.036007
Published: 11 March 2015
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Cited by 17 scholarly publications and 2 patents.
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KEYWORDS
In vivo imaging
Blood
Photoacoustic microscopy
Photoacoustic spectroscopy
Sodium
Absorption
Blood vessels
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