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15 November 2018 Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography
Yong Da Sie, Chia-Yuan Chang, Chun-Yu Lin, Nan-Shan Chang, Paul J. Campagnola, Shean-Jen Chen
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Journal of Biomedical Optics, Vol. 23, Issue 11, 116502 (November 2018). https://doi.org/10.1117/1.JBO.23.11.116502
Abstract
Conventional temporal focusing-based multiphoton excitation microscopy (TFMPEM) can offer widefield optical sectioning with an axial excitation confinement of a few microns. To improve the axial confinement of TFMPEM, a binary computer-generated Fourier hologram (CGFH) via a digital-micromirror-device (DMD) was implemented to intrinsically improve the axial confinement by filling the back-focal aperture of the objective lens. Experimental results show that the excitation focal volume can be condensed and the axial confinement improved about 24% according to the DMD holography. In addition, pseudouniform MPE can be achieved using two complementary CGFHs with rapid pulse-width modulation switching via the DMD. Furthermore, bioimaging of CV-1 in origin with SV40 genes-7 cells demonstrates that the TFMPEM with binary DMD holography can improve image quality by enhancing axial excitation confinement and rejecting out-of-focus excitation.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2018/$25.00 © 2018 SPIE
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Yong Da Sie, Chia-Yuan Chang, Chun-Yu Lin, Nan-Shan Chang, Paul J. Campagnola, and Shean-Jen Chen "Fast and improved bioimaging via temporal focusing multiphoton excitation microscopy with binary digital-micromirror-device holography," Journal of Biomedical Optics 23(11), 116502 (15 November 2018). https://doi.org/10.1117/1.JBO.23.11.116502
Received: 28 June 2018; Accepted: 25 October 2018; Published: 15 November 2018
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Cited by 9 scholarly publications and 1 patent.
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KEYWORDS
Digital micromirror devices
Binary data
Diffraction
Objectives
Holography
Microscopy
Multiphoton microscopy
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