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27 February 2018 Topography and refractometry of sperm cells using spatial light interference microscopy
Lina Liu, Mikhail E. Kandel, Marcello Rubessa, Sierra Schreiber, Matthew B. Wheeler, Gabriel Popescu
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Journal of Biomedical Optics, Vol. 23, Issue 2, 025003 (February 2018). https://doi.org/10.1117/1.JBO.23.2.025003
Abstract
Characterization of spermatozoon viability is a common test in treating infertility. Recently, it has been shown that label-free, phase-sensitive imaging can provide a valuable alternative for this type of assay. We employ spatial light interference microscopy (SLIM) to perform high-accuracy single-cell phase imaging and decouple the average thickness and refractive index information for the population. This procedure was enabled by quantitative-phase imaging cells on media of two different refractive indices and using a numerical tool to remove the curvature from the cell tails. This way, we achieved ensemble averaging of topography and refractometry of 100 cells in each of the two groups. The results show that the thickness profile of the cell tail goes down to 150 nm and the refractive index can reach values of 1.6 close to the head.
© 2018 Society of Photo-Optical Instrumentation Engineers (SPIE) 1083-3668/2018/$25.00 © 2018 SPIE
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Lina Liu, Mikhail E. Kandel, Marcello Rubessa, Sierra Schreiber, Matthew B. Wheeler, and Gabriel Popescu "Topography and refractometry of sperm cells using spatial light interference microscopy," Journal of Biomedical Optics 23(2), 025003 (27 February 2018). https://doi.org/10.1117/1.JBO.23.2.025003
Received: 18 November 2017; Accepted: 23 January 2018; Published: 27 February 2018
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Cited by 16 scholarly publications and 1 patent.
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KEYWORDS
Refractive index
Microscopy
Refractometry
Phase shifts
Neck
Phase shift keying
Spatial light modulators
CHORUS Article. This article was made freely available starting 27 February 2019
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