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12 July 2019 Excitation of erbium-doped nanoparticles in 1550-nm wavelength region for deep tissue imaging with reduced degradation of spatial resolution
Masahito Yamanaka, Hirohiko Niioka, Taichi Furukawa, Norihiko Nishizawa
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Journal of Biomedical Optics, Vol. 24, Issue 7, 070501 (July 2019). https://doi.org/10.1117/1.JBO.24.7.070501
Abstract
Rare-earth-doped nanoparticles are one of the emerging probes for bioimaging due to their visible-to-near-infrared (NIR) upconversion emission via sequential single-photon absorption at NIR wavelengths. The NIR-excited upconversion property and high photostability make this probe appealing for deep tissue imaging. So far, upconversion nanoparticles include ytterbium ions (Yb3  +  ) codoped with other rare earth ions, such as erbium (Er3  +  ) and thulium (Tm3  +  ). In these types of upconversion nanoparticles, through energy transfer from Yb3  +   excited with continuous wave light at a wavelength of 980 nm, upconversion emission of the other rare earth dopants is induced. We have found that the use of the excitation of Er3  +   in the 1550-nm wavelength region allows us to perform deep tissue imaging with reduced degradation of spatial resolution. In this excitation–emission process, three and four photons of 1550-nm light are sequentially absorbed, and Er3  +   emits photons in the 550- and 660-nm wavelength regions. We demonstrate that, compared with the case using 980-nm wavelength excitation, the use of 1550-nm light enables us to moderate degradation of spatial resolution in deep tissue imaging due to the lower light scattering coefficient compared with 980-nm light. We also demonstrate that live cell imaging is feasible with this 1550 nm excitation.
CC BY: © The Authors. Published by SPIE under a Creative Commons Attribution 4.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.
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Masahito Yamanaka, Hirohiko Niioka, Taichi Furukawa, and Norihiko Nishizawa "Excitation of erbium-doped nanoparticles in 1550-nm wavelength region for deep tissue imaging with reduced degradation of spatial resolution," Journal of Biomedical Optics 24(7), 070501 (12 July 2019). https://doi.org/10.1117/1.JBO.24.7.070501
Received: 29 April 2019; Accepted: 24 June 2019; Published: 12 July 2019
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Cited by 11 scholarly publications.
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KEYWORDS
Nanoparticles
Luminescence
Erbium
Deep tissue imaging
Near infrared
Spatial resolution
Upconversion
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