Dr. Ali Öztuna Profile

Dr. Ali Öztuna

at Gulhane Military Medical Academy

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Proceedings Article | 5 May 2012 Paper

Testing and comparison of the coating materials for immunosensors on QCM

Ali Oztuna, Hasan Nazir

Proceedings Volume 8358, 83580J (2012) https://doi.org/10.1117/12.918450

KEYWORDS: Crystals, Scanning electron microscopy, Sensors, Quartz, FT-IR spectroscopy, Coating, Gold, Photomicroscopy, Polymers, Liquids

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In immunoassay based biosensors development studies polymers, as a matrix, and thiol, amine and aldehyde derivative compounds, as a antibody linker, are to be experimented. Aim of this study is to test amine and acetate functional group containing derivatives in liquid phase in order to develop an antibody immobilization strategy for Quartz Crystal Microbalance (QCM) system. In our study, 4-aminothiophenol (4-AT), carboxylated-PVC (PVC-COOH) and aminated- PVC (PVC-NH₂) compared with each other as a coating material. Surface of the coated AT-cut gold crystals were characterized with Fourier Transform Infrared spectrometry (FTIR) and Scanning Electron Microscobe (SEM) and tested in a Bacillus anthracis (GenBank: GQ375871) spores immunoassay model system. Subsequently, a series of SEM micrographs were taken again in order to investigate surface morphology and show the presence of the B. anthracis spores on the sensor surface. When experimental results and SEM images were evaluated together, it was suggested that with the synthesis of PVC like open-chained polymers, containing -NH₂ and -SH functional groups, B. anthracis spore detection can be accomplished on QCM without requiring complicated immobilization procedures and expensive preliminary preparations.

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