We explored the capabilities of quantitative phase imaging (QPI) with digital holographic microscopy (DHM) to quantify nanoparticle-induced tissue alterations and the performance of DHM QPI for imaging hematoxylin-eosin-stained samples. In a pilot study, paraffin and cryosections from nanomaterial-laden rat lungs and vehicle-treated control tissue were analyzed. Our results from rat lungs show that the tissue average refractive index and morphology parameters extracted from QPI images allow the quantification of visible tissue changes due to toxic nanomaterials. Moreover, our results demonstrate that DHM with near-infrared laser light enables high quality QPI of samples with common histology staining.
In laser based digital holographic microscopy (DHM) quantitative phase imaging (QPI) is affected by scattering and interference fringes due to internal reflections in the experimental setup. We present a concept for the reduction of such coherence induced disturbances. In our approach, the sample illumination light is modulated by an electrically focus tunable lens while series of digital off-axis holograms are recorded from which subsequently averaged QPI images are calculated. The concept is compatible with Mach-Zehnder interferometer-based off-axis DHM and capable for usage with commercial research microscopes. The performance is illustrated by results from living cells.
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