Mr. Bartłomiej Bałamut Profile

Bartłomiej Bałamut

at Internatinal Ctr. for Translational Eye Research

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Author

Publications (3)

SPIE Journal Paper | 8 October 2024 Open Access

In vivo volumetric analysis of retinal vascular hemodynamics in mice with spatio-temporal optical coherence tomography

Piotr Węgrzyn, Wiktor Kulesza, Maciej Wielgo, Sławomir Tomczewski, Anna Galińska, Bartłomiej Bałamut, Katarzyna Kordecka, Onur Cetinkaya, Andrzej Foik, Robert Zawadzki, Dawid Borycki, Maciej Wojtkowski, Andrea Curatolo

NPh, Vol. 11, Issue 04, 045003, (October 2024) https://doi.org/10.1117/12.10.1117/1.NPh.11.4.045003

KEYWORDS: Eye, Hemodynamics, Retina, Imaging systems, In vivo imaging, Cameras, Optical coherence tomography, Signal to noise ratio, Neurophotonics, Tunable filters

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SignificanceMicrocirculation and neurovascular coupling are important parameters to study in neurological and neuro-ophthalmic conditions. As the retina shares many similarities with the cerebral cortex and is optically accessible, a special focus is directed to assessing the chorioretinal structure, microvasculature, and hemodynamics of mice, a vital animal model for vision and neuroscience research.AimWe aim to introduce an optical imaging tool enabling in vivo volumetric mouse retinal monitoring of vascular hemodynamics with high temporal resolution.ApproachWe translated the spatio-temporal optical coherence tomography (STOC-T) technique into the field of small animal imaging by designing a new optical system that could compensate for the mouse eye refractive error. We also developed post-processing algorithms, notably for the assessment of (i) localized hemodynamics from the analysis of pulse wave–induced Doppler artifact modulation and (ii) retinal tissue displacement from phase-sensitive measurements.ResultsWe acquired high-quality, in vivo volumetric mouse retina images at a rate of 113 Hz over a lateral field of view of ∼500 μm. We presented high-resolution en face images of the retinal and choroidal structure and microvasculature from various layers, after digital aberration correction. We were able to measure the pulse wave velocity in capillaries of the outer plexiform layer with a mean speed of 0.35 mm/s and identified venous and arterial pulsation frequency and phase delay. We quantified the modulation amplitudes of tissue displacement near major vessels (with peaks of 150 nm), potentially carrying information about the biomechanical properties of the retinal layers involved. Last, we identified the delays between retinal displacements due to the passing of venous and arterial pulse waves.ConclusionsThe developed STOC-T system provides insights into the hemodynamics of the mouse retina and choroid that could be beneficial in the study of neurovascular coupling and vasculature and flow speed anomalies in neurological and neuro-ophthalmic conditions.

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Proceedings Article | 13 March 2024 Poster

Monitoring hemodynamics in the mouse retina via ultrafast volumetric spatio-temporal optical coherence tomography (STOC-T)

Wiktor Kulesza, Maciej Wielgo, Piotr Węgrzyn, Sławomir Tomczewski, Onur Centinkaya, Katarzyna Kordecka, Anna Galińska, Bartłomiej Bałamut, Egidijus Auksorius, Andrzej Foik, Robert Zawadzki, Maciej Wojtkowski, Dawid Borycki, Andrea Curatolo

Proceedings Volume PC12824, PC128241P (2024) https://doi.org/10.1117/12.3005418

KEYWORDS: Optical coherence tomography, Hemodynamics, Retina, Ultrafast phenomena, Optical sensing, Nerve, Blood, Wave propagation, Ultrafast imaging, Tissues

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Proceedings Article | 11 August 2023 Paper

Ultrafast volumetric imaging with a spatio-temporal optical coherence tomography (STOC-T) system for monitoring hemodynamics in mouse retinal vessels directly from structural data

Wiktor Kulesza, Maciej Wielgo, Piotr Węgrzyn, Sławomir Tomczewski, Onur Cetinkaya, Katarzyna Kordecka, Anna Galińska, Bartłomiej Bałamut, Egidijus Auksorius, Andrzej Foik, Robert Zawadzki, Maciej Wojtkowski, Dawid Borycki, Andrea Curatolo

Proceedings Volume 12632, 126320O (2023) https://doi.org/10.1117/12.2670568

KEYWORDS: Imaging systems, Hemodynamics, Optical coherence tomography, Ultrafast imaging, Eye, Retina, Optical sensing, Crosstalk, Cameras, Ultrafast phenomena

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