Mr. David Alan Bergstein Profile

David Alan Bergstein

at Boston Univ

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Proceedings Article | 4 October 2007 Paper

Hyperspectral Fourier transform spectrometer for spectral self-interference measurements of biological material on surfaces

Julia Dupuis, I. Emre Ozkumur, David Bergstein, James Needham, Anna Swan, Bennett Goldberg, Michael Ruane, M. Selim Unlu, James Engel, David Carlson

Proceedings Volume 6759, 675904 (2007) https://doi.org/10.1117/12.733203

KEYWORDS: Fourier transforms, Mirrors, Interferometers, Signal to noise ratio, Spectroscopy, Luminescence, Microscopy, Reflectance spectroscopy, CCD cameras, Biomedical optics

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A hyperspectral Fourier transform spectrometer (HS-FTS) has been developed to study biological material binding to surfaces through spatially resolved, spectral self-interference fluorescence microscopy and also label-free white light reflectance spectroscopy. Spectral self-interference fluorescence microscopy yields the height of fluorescent tags bound to a specific location on biomolecules tethered to a surface, and from this the biomolecule conformation can be predicted; white light reflectance spectroscopy yields the average height of an ensemble of biomolecules relative to the surface. The HS-FTS is composed of a small, step scanning Michelson interferometer made by Optra, Inc., a series of commercial off the shelf imaging lenses, and a 12-bit thermoelectrically-cooled CCD camera. The system operates over the 500 to 900 nm spectral range with user defined spectral resolution, thereby supporting use of a host of fluorescent tags or white light spectral windows. The system also supports near real-time hyperspectral cube acquisition via undersampling with the use of a spectral filter and user defined interferometer step increments. The overall approach offers flexible yet sensitive measurement capability for a variety of biological studies. Preliminary results are presented of both spectral self-interference fluorescence microscopy and white light reflectance spectroscopy measurements of artificial, photographically etched surfaces with feature heights on the order of 10 nm. Planned future work includes spectral self-interference fluorescence microscopy measurements of biomolecule conformation as manipulated by external electrical and magnetic fields as well as label-free white light reflectance spectroscopy measurements of DNA microarrays.

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