We describe a double optical trap for micromanipulation of
biomolecules. This set-up allows to bring a DNA molecule grafted
between two beads in contact with microelectrodes, in order to
perform electrical measurements at the single molecule level in a
well-controlled geometry.
The use of an opaque surface like silicon compatible with
lithography imposes to work in a reflection configuration with
the same objective for trapping and imaging the bead. The
experimental constraints raised by this optical configuration are
detailed.
By combining drag force and optical trapping, we are able to trap
separately the two beads and to perform force versus extension
measurements in this reflection configuration. Finally, deposition
of one stretched DNA molecule on Au electrodes fabricated by
electron beam lithography on silicon wafers is shown.
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