Cancer cells in the hypoxic environment alter their metabolism to address the low oxygen stress. Such metabolic changes contribute to cancer resistance to chemotherapy and radiotherapy treatment. Understanding the hypoxia-induced metabolic changes allows for finding new targets to treat hypoxic cancer cells. We applied stimulated Raman scattering spectroscopic imaging to explore the metabolic changes of cancer cells exposed to hypoxia. We found spatial redistribution and content changes of lipid droplets (LDs) in various cancer types. We also studied the sources contributing to the formation of LDs and discovered the relationship between LD formation and endoplasmic reticulum stress.
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