Light scattering patterns have been calculated for a simple optical model constructed for single smooth and nonsmooth
lymphocytes of healthy and infected individuals. It has been shown that the smooth and nonsmooth cells can be resolved
using the intensities of the sideward and backward scattered light. We have found by calculations and validated by the
flow-cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a
preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flowcytometry
detecting of individuals infected with viruses of hepatitises B or C and some others viruses are presented.
A simple optical model of single lymphocytes with smooth and nonsmooth surfaces has been developed for healthy and infected individuals. The model can be used for rapid (in the real-time scale) solution of the inverse light-scattering problem on the basis of optical data measured by label-free flow cytometry. Light scattering patterns have been calculated for the model developed. It has been shown that the smooth and nonsmooth cells can be resolved using the intensities of the sideward- and backward-scattered light. We have found by calculations and validated by the flow cytometer experiments that intensity distributions for the cells of lymphocyte populations can be used as a preliminary signatures of some virus infections. Potential biomedical applications of the findings for label-free flow cytometry detection of individuals infected with viruses of hepatitises B or C and some others viruses are presented.
Cell structure, shape, and size distribution are characterized by optical measurement of normal peripheral blood
granulocytes in a cell suspension as applied to a cell discrimination by the solution of the direct and inverse light-scattering
problems in scanning flow cytometry (SFC). The distribution laws of the cell and nuclei sizes are estimated. A
morphometric model of a segmented neutrophilic granulocyte is constructed on the base of the gained data. An
interrelation between the cell and nucleus metric characteristics is deduced. The obtained interrelation allows decreasing
a number of independent parameters of the cell model to adapt it to the real-time discrimination of the cells. The findings
can be used as input parameters for the solution of the direct and inverse light-scattering problems in SFC-dispensing
with a costly and time-consuming immunophenotyping of the cells.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.