Quantification of fibrosis is critical for the management of inflammatory bowel disease. In this study, two measurements, collagen-to-Hb ratio quantified by spectroscopic analysis and tissue stiffness quantified by PA-strain, measured by our PA-US balloon catheter were employed to quantify intestinal fibrosis in 23 rabbits in vivo. Results show that both measurements can distinguish the low histological fibrosis (0-2) and high histological fibrosis (3-5) with statistical significance (p-value<0.001). Collagen-to-Hb ratio and PA-strain are highly correlated with the fibrosis stages with correlation of 0.67 and 0.64, respectively. PA-strain is positively correlated to Young’s Modulus measured ex vivo using microelastometer with correlation 0.81.
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