We assessed the spectral sensitivity of the pupillary light reflex in mice using a high power super continuum white light (SCWL) source in a dual wavelength configuration. This novel approach was compared to data collected from a more traditional setup using a Xenon arc lamp fitted with monochromatic interference filters. Irradiance response curves were constructed using both systems, with the added benefit of a two-wavelength, equivocal power, output using the SCWL. The variables applied to the light source were intensity, wavelength and stimulus duration through which the physiological output measured was the minimum pupil size attained under such conditions. We show that by implementing the SCWL as our novel stimulus we were able to dramatically increase the physiological usefulness of our pupillometry system.
We present the use of a prism as an imaging adjunct with a multimodal system of optical coherence tomography and
confocal microscopy operating at 1320 nm and 970 nm respectively. A comparison is performed between en-face OCT
images acquired using the system and cross section OCT images obtained through a prism inserted into neuronal tissue
of an intact ex-vivo murine brain. The en-face images and cross section images are scans of the same area; however each
method has shown different aspects, allowing for greater interpretation of the neuronal tissue.
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