We observed the changes in fluorescence intensity which was based on fluorescent protein (mCherry) when the fluorescent protein expressed E-coli attached on the natural and artificial nanostructural surface. Time-lapse imaging was used to analyze the changes in fluorescence intensity caused by the effusion of intercellular fluid, including fluorescent protein, at the single cell level. We found that there were three stages of changes in the fluorescence intensity gradient. These characteristics were observed both on the natural and artificial nanostructural surface.
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