Dr. John S. Oakey Profile

Dr. John S. Oakey

at Metafluidics Inc

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Proceedings Article | 18 October 2004 Paper

Optical trapping for complex fluid microfluidics

Tor Vestad, John Oakey, David Marr

Proceedings Volume 5514, (2004) https://doi.org/10.1117/12.560152

KEYWORDS: Microfluidics, Optical tweezers, Particles, Mirrors, Biological research, Resistance, Blood, Diagnostics, Diffusion, Capillaries

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Many proposed applications of microfluidics involve the manipulation of complex fluid mixtures such as blood or bacterial suspensions. To sort and handle the constituent particles within these suspensions, we have developed a miniaturized automated cell sorter using optical traps. This microfluidic cell sorter offers the potential to perform chip-top microbiology more rapidly and with less associated hardware and preparation time than other techniques currently available. To realize the potential of this technology in practical clinical and consumer lab-on-a-chip devices however, microscale control of not only particulates but also the fluid phase must be achieved. To address this, we have developed a mechanical fluid control scheme that integrates well with our optical separations approach. We demonstrate here a combined technique, one that employs both mechanical actuation and optical trapping for the precise control of complex suspensions. This approach enables both cell and particle separations as well as the subsequent fluid control required for the completion of complex analyses.

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