Optical diffraction tomography has recently emerged as a promising technique for obtaining morphological and biochemical information, such as lipid and protein concentrations, from individual cells. Herein, we propose a quantitative phase imaging application that enables the measurement of morphological and physiological parameters in asthma-specific immune cells without the need for exogenous labeling agents. By analyzing the reconstructed 3D refractive index distribution, our technique provides quantitative data on asthma-specific cell physiology. The potential of these imaging-based biomarkers as tools for characterizing phenotypical changes in immune cells may support the pursuit of early diagnosis and a comprehensive understanding of asthma diseases.
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