We present a straightforward approach to making sensitive fluorescence measurements using a chopped laser and phase-lock detection scheme. By using high-quality optical filters in the excitation and detection paths, the trade-off between spectral information and ultimate sensitivity is controlled by the choice of filter edge locations and bandwidths. Using this approach, we demonstrate a sensitivity of ~1 pW optical power of detected fluorescence over a bandwidth of ~125 nm centered at 505 nm using a 405 nm diode laser as an excitation source. We present the details of the measurement technique and discuss its value in selection of optical materials for fluorescence-based analytical systems.
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