This work presents micro-grids integrated to cell culture boxes. These grids allow the systematic registration of
the position of a zone observed by optical microscopy in a such way that it is possible to find it again easily for
new observations for instance after culture on drug injection. The position knowledge allows also the numerical
superimposition of recorded images in a common position reference system with a sub-pixel precision. It become
thus straightforward to perform a site by site analysis of the possible evolutions that may have occurred in the
biological medium.
A vision system is used for measuring in-plane target displacement, position and orientation. Pseudo-periodic
patterns fixed on the target forms a phase reference. Absolute position is determined with subpixel accuracy by
phase computations. Various position encoding designs are proposed for different displacement ranges and resolution.
Performances obtained are compared and discussed for both displacement and orientation measurements.
The capability to resolve position on depth ranges larger than the lens depth of focus is demonstrated.
The reconstruction of the object surface with inherently discontinuous regions is usually a difficult problem by using
standard phase unwrapping technique i.e. the spatial unwrapping. To recover the range data of such a surface, Huntley
and Saldner recently proposed a temporal phase unwrapping procedure (TPUP) and Peng X. et al. based on their work
proposed an enhanced recursive scheme for the TPUP. However the phase jumps no necessarily they correspond to
multiples of 2pi exactly. Due to this, an enhanced scheme for temporal unwrapping in order to increase its efficiency is
introduced. Additionally, we present some results obtained by mean of temporal unwrapping and spatial unwrapping, in
order to compare them and to demonstrate the effectiveness of the temporal unwrapping.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.