Rapid substance screening is a vital yet difficult task. Different materials are best analysed by different techniques, and therefore a single tool may not always be able to identify an unknown substance. This can be addressed by a multimodal approach, simultaneously combining several orthogonal techniques into a single tool. Here we demonstrate a proof-of-concept for a solution based on three complementing techniques - infrared spectroscopy, ultraviolet fluorescence spectroscopy and microscopic imaging – for rapid acquisition of a rich dataset well suited for biochemical samples classification, with dedicated signal processing extracting the descriptive features and identifying the nature of the material.
The dual requirement for high spatial and substance specificity makes stand-off in-theatre biological detection of surface biological contaminants extremely challenging. We will describe a novel combined fluorescence multispectral imaging (MSI) and stand-off Raman approach which are united through their use of deep-UV (sub-250 nm excitation. This allows high-confidence location and classification of candidate contamination sites over the camera field of view, and subsequent resonance-Raman classification of these identified sites. Stand-off Raman is enabled through the use of a novel, extremely high-throughput Spatial Heterodyne spectrometer. The viability of this approach is confirmed through its use on application relevant biological simulant samples.
Access to the requested content is limited to institutions that have purchased or subscribe to SPIE eBooks.
You are receiving this notice because your organization may not have SPIE eBooks access.*
*Shibboleth/Open Athens users─please
sign in
to access your institution's subscriptions.
To obtain this item, you may purchase the complete book in print or electronic format on
SPIE.org.
INSTITUTIONAL Select your institution to access the SPIE Digital Library.
PERSONAL Sign in with your SPIE account to access your personal subscriptions or to use specific features such as save to my library, sign up for alerts, save searches, etc.