KEYWORDS: Cartilage, Raman spectroscopy, In vivo imaging, Tissues, Tissue engineering, Systems modeling, Non-invasive medical diagnostics, Magnetic resonance imaging, Collagen
Cartilage tissue engineering (TE) is a promising osteoarthritis treatment strategy, whereby cellularized tissue constructs are implanted in cartilage defect sites to promote tissue repair. A significant impediment to developing effective TE strategies is a lack of in vivo diagnostic platforms to monitor engineered cartilage growth. Here, we utilize an ex vivo model system of chondrocyte-seeded tissue constructs, demonstrating the capability of a novel Raman arthroscopic-configured needle probe to monitor the composition and material properties of developing TE cartilage over time. This work supports Raman arthroscopy as a clinical tool for monitoring TE cartilage, providing objective assessments of TE platform efficacies.
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