There are gaps in our understanding of the neural signatures underlying naturalistic deficits associated with impaired motor imitation in autism spectrum disorder (ASD) due to logistical limitations in neuroimaging modalities like fMRI. Therefore, we utilized high-density diffuse optical tomography (HD-DOT) to image twenty-three young adults as they observed and imitated sequences of upper extremity movements. Alterations in multiple cortical areas were observed when comparing neural responses to motor observation and motor imitation in this sample. This establishes the utility of HD-DOT for neuroimaging during naturalistic overt motion
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