Mr. Nicola Moscelli Profile

Nicola Moscelli

at Technische Univ Wien

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Proceedings Article | 5 May 2011 Paper

In incubator live cell imaging platform

Nicola Moscelli, Wojciech Witarski, Sander van den Driesche, Michael Vellekoop

Proceedings Volume 8066, 80661M (2011) https://doi.org/10.1117/12.886908

KEYWORDS: Sensors, Image sensors, Live cell imaging, Light emitting diodes, Charge-coupled devices, CCD image sensors, Carbon dioxide, Statistical analysis, Actuators, Imaging systems

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In this contribution we present an imaging platform that operates in standard incubators, allowing the investigation of multiple cell cultures at the same time. Also, the sensor platform operates with disposable multi-well plates. The system consists of four image sensors (Charge-Coupled Devices) built into a custom made holder, in which a multi-well plate can be positioned without the need of further alignment. The focal points of the image sensors are fixed at the bottom of the wells of interest. Above the multi-well plate, white light emitting LEDs with an aperture are mounted to provide orthogonal illumination over the sensors. For the observation and understanding of tumour progression, cell proliferation and cell motility studies are of great importance. For such studies, a sensor system that monitors cell motility in four wells simultaneously (of a 24-well plate) has been designed and realized. In the present work we focus on measuring the motility of adherently grown mammalian epithelial cells. Simultaneous, real-time observation of stimulated (with hepatocyte growth factor) and control samples of MDCK (Madin-Darby Canine Kidney) cells has been carried out. The presented imaging platform is an attractive and versatile alternative to conventional time-lapse microscopy to monitor the motility of individual cells. Furthermore, the high-throughput feature makes its use advantageous for the simultaneous tracking of biological samples under different conditions.

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