The results of expanded spectral analysis of donor dentin materials with the use of Raman spectroscopy method are presented in the work. The mathematical methods of improving the resolution of spectral contours and the chemometric analysis were used for estimation of the component composition of dentin materials during their demineralization.
The results of studies of the enamel of teeth with periodontitis using the Raman spectroscopy method are presented in the work. The criteria for noninvasive diagnosing of periodontitis were developed. It is shown that this method allows noninvasive rapid assessment of periodontitis on the base of change of tooth enamel spectral properties.
The results of experimental study of samples of dental tissues affected by periodontitis using Raman spectroscopy method are presented in the work. Spectral changes are detected in hard dental tissues affected by periodontitis. The main spectral changes are seen in the wavenumbers of 956 cm-1, 1236 cm-1, 1269cm-1 and 1663 cm-1, owing to the change of mineral-protein components in case of periodontitis.
The results of expanded spectral analysis of donor bone implants using the Raman spectroscopy method are presented in the work. Mathematical methods of improving resolution of spectral contours and chemometric analysis are used for evaluation of component composition of the implants. It is shown that the Raman spectroscopy can be used for evaluation of relative concentration of mineral and organic components in extracellular matrix of bone tissue. Spectral features of the samples of bone tissues demineralized and processed with ultrasound with different degree are found as a result of the study. The criteria allowing evaluating the component composition during manufacture of bioimplants are suggested in the work.
The paper presents the results of extended spectral analysis of bone tissue in osteoporosis and evaluation of the effectiveness of correction of this pathological condition using allogeneic hydroxyapatite (HAP). It is shown that the optical method allows to determine the most significant criteria for evaluating the effectiveness of correction using allogeneic hydroxyapatite of different concentrations.
The spectral study results and comparative evaluation of the component composition of the surfaces of hard palate implant samples made by the technology "Lioplast", used in the clinic in the field of atrophic processes in multiple recessions of the gums, using the method of Raman spectroscopy (RS) are presented. It is shown that Raman spectroscopy can be used to assess changes in the composition of implants based on the hard palate during their processing. The coefficients were introduced and a two-dimensional analysis was carried out, which showed that the main components are preserved and DNA/RNA is removed during processing, which improves the quality of the material that provides the possibility of a good clinical effect in the treatment of multiple recessions of the gums.
This paper presents the results of the experimental study of the use of ultrasonic treatment for the production of bone implants using Raman spectroscopy (RS). It shows that Raman spectroscopy can be used to estimate a change of bone implants composition during their processing. It was shown that ultrasonic treatment reduces the concentration of cellular components in the bone tissue. The conclusion was based on the two-dimensional analysis of the given ratios.
The results of application of the Raman spectroscopy method for the estimation of alternative sources for the production of bone spongy implants using the "Lioplast" technology, namely, the femoral heads resected in the operation of hip replacement surgery, are presented. It is shown that Raman spectroscopy can be used to estimate the component composition of the surface of bone implants during their processing. Comparing different sources of sponge bone production, no significant differences were found, but there are differences in the ratio of the intensities of the Raman peaks at wave numbers 1555 cm-1 and 1665 cm-1 corresponding to amide II and amide I, and also in the intensity of Raman peaks at wave numbers of 959 cm-1 (РО43- (ν1)) and 1068 cm-1 (СO32- (ν1) B-type substitution).
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