We have developed a novel OCT needle probe appropriate for imaging deep within muscle and assessed its ability to quantify intramuscular fat. The OCT needle probe is designed to operate in common-path mode, removing the need for a pathlength-matched reference arm. Scanning is performed by manually inserting and removing the needle from the muscle, using a speckle decorrelation algorithm to correct for variation in the speed at which the needle is moved. Four OCT needle probes were integrated into a single handpiece for simultaneous measurement at different locations in the muscle and multiplexed to a single OCT scanner. The OCT data is segmented using a convolutional neural network, automatically quantifying the percentage of intramuscular fat. The system was assessed on muscle from 275 lambs carcases and measured the percentage of intramuscular fat with an average absolute error of 0.65%.
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