Ms. Sabrina Civale Profile

Sabrina Civale

at Northeastern Univ

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Proceedings Article | 5 March 2021 Presentation

Fitting approach for eliminating scattered light

Sabrina Civale, Chi Zhang, Yao Wang, Samuel Chung

Proceedings Volume 11649, 116490E (2021) https://doi.org/10.1117/12.2585518

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Unwanted background fluorescence in microscopy can occur when light from fluorescent structures scatters against nearby tissues. Bright cell bodies produce haze that mask nearby dim structures, including neuronal fibers. We have developed a method to eliminate this haze by fitting it to a simple function. This method enables clearer post-imaging visualization of axons in our in vivo imaging of neurons. We are investigating ways to increase the computation speed of our background fitting technique to allow for real-time image improvement. The implementation of our method in real-time imaging will broaden the potential applications of this fitting method.

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