Sensing at the single cell level can provide insights into its dynamics and heterogeneity, yielding information otherwise unattainable with traditional biological methods where average population behavior is observed. In this context, optical tweezers provide the ability to select, separate, manipulate and identify single cells or other types of microparticles, potentially enabling single cell diagnostics. Forward or backscatter analysis of the light interacting with the trapped cells can provide valuable insights on the cell optical, geometrical and mechanical properties. In particular, the combination of tweezers systems with advanced machine learning algorithms can enable single cell identification capabilities. However, typical processing pipelines require a training stage which often struggles when trying to generalize to new sets of data. In this context, fully automated tweezers system can provide mechanisms to obtain much larger datasets with minimum effort form the users, while eliminating procedural variability. In this work, a pipeline for full automation of optical tweezers systems is discussed. A performance comparison between manually operated and fully automated tweezers systems is presented, clearly showing advantages of the latter. A case study demonstrating the ability of the system to discriminate molecular binding events on microparticles is presented.
Lately, the field of optical computing resurfaced with the demonstration of a series of novel photonic neuromorphic schemes for autonomous and inline data processing promising parallel and light-speed computing. We emphasize the Photonic Extreme Learning Machine (PELM) as a versatile configuration exploring the randomness of optical media and device production to bypass the training of the hidden layer. Nevertheless, the implementation of this framework is limited to having the output layer performed digitally. In this work, we extend the general PELM implementation to an all-optical configuration by exploring the amplitude modulation from a spatial light modulator (SLM) as an output linear layer with the main challenge residing in the training of the output weights. The proposed solution explores the package pyTorch to train a digital twin using gradient descent back-propagation. The trained model is then transposed to the SLM performing the linear output layer. We showcase this methodology by solving a two-class classification problem where the total intensity reaching the camera predicts the class of the input sample.
This communication explores an optical extreme learning architecture to unravel the impact of using a nonlinear optical media as an activation layer. Our analysis encloses the evaluation of multiple parameters, with special emphasis on the efficiency of the training process, the dimensionality of the output space, and computing performance across tasks associated with the classification in low-dimensionality input feature spaces. The results enclosed provide evidence of the importance of the nonlinear media as a building block of an optical extreme learning machine, effectively increasing the size of the output space, the accuracy, and the generalization performances. These findings may constitute a step to support future research on the field, specifically targeting the development of robust general-purpose all-optical hardware to a full-stack integration with optical sensing devices toward edge computing solutions.
In this work we use the concept of paraxial fluids of light to explore quantum turbulence, probing a turbulent regime induced on an optical beam propagating inside a defocusing nonlinear media. For that purpose, we establish a physical analogue of a two-component quantum fluid by making use of orthogonal polarizations and incoherent beam interaction, obtaining a system for which the perturbative excitations follow a modified Bogoliubov-like dispersion relation. This dispersion relation features regions of instability that define an effective range of energy injection and that are easily tuned by manipulating the relative angle of incidence between the two components. Our numerical results support the predictions and show evidence of direct and inverse turbulent cascades expected from weak wave turbulence theories, which may inspire new ways to explore to quantum turbulence with optical analogues.
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