Fluorescent Light-inducible Proteins are proteins of interest that have Dronpa domains fused to both termini. In the dark, the Dronpa domains tetramerize and cage the protein, but light induces Dronpa dissociation and activates the protein. Here, we describe four experimental systems for light activation of mammalian cells expressing FLiPs.
KEYWORDS: Sensors, Semiconductor lasers, Absorption, Temperature metrology, Combustion, Environmental sensing, Vertical cavity surface emitting lasers, Cesium, Temperature sensors, Control systems
Diode laser absorption offers the possibility of high-speed, robust, and rugged sensors for a wide variety of practical applications. Pressure broadening complicates absorption measurements of gas temperature and species concentrations in high-pressure, high-temperature practical environments. More agile wavelength scanning can enable measurements of temperature and species concentrations in flames and engines as demonstrated by example measurements using wavelength scanning of a single DFB in laboratory flames or a vertical cavity surface emitting laser (VCSEL) in a pulse detonation engine environment. Although the blending of multiple transitions by pressure broadening complicates the atmospheric pressure spectrum of C2H4 fuel, a scanned wavelength strategy enables quantitative measurement of fuel/oxidizer stoichiometry. Wavelength-agile scanning techniques enable high-speed measurements in these harsh environments.
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