Fast detection of bacterial concentrations is important for the food industry and for healthcare. Early detection of
infections and appropriate treatment is essential since, the delay of treatments for bacterial infections tends to be
associated with higher mortality rates. In the food industry and in healthcare, standard procedures require the count of
colony-forming units in order to quantify bacterial concentrations, however, this method is time consuming and reports
require three days to be completed. An alternative is metabolic-colorimetric assays which provide time efficient in vitro
bacterial concentrations. A colorimetric assay based on Resazurin was developed as a time kinetic assay (KRA) suitable
for bacterial concentration measurements. An optimization was performed by finding excitation and emission
wavelengths for fluorescent acquisition. A comparison of two
non-related bacteria, foodborne pathogens Escherichia coli
and Listeria monocytogenes, was performed in 96 well plates. A metabolic and clonogenic dependence was established
for fluorescent kinetic signals.
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