Longitudinal characterisation of the tumour vascular response to radiotherapy is essential for understanding the role of oxygenation and microvascular disruption in response to therapy. Using multi-scale in vivo photoacoustic imaging (PAI), we assessed early response to two hypofractionated radiotherapy schemes in two human breast cancer models. Mesoscopic and multispectral tomographic photoacoustic imaging was performed 24h pre-, post-radiotherapy, and at endpoint. PAI biomarkers were validated ex vivo with multiplex immunofluorescence using a 20-plex panel developed specifically for vascular response assessment at sub-cellular resolution. PAI captured radiotherapy response, revealing the differential effect between radiotherapy schemes and models with different hypoxia phenotypes.
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