Hadamard-transform spectral acquisition in a broadband stimulated Raman scattering microscope with an acousto-optical tunable filter

Luca Genchi; Andrea Bucci; Siarhei Laptenok; Carlo Liberale

doi:10.1117/12.2577530

5 March 2021 Hadamard-transform spectral acquisition in a broadband stimulated Raman scattering microscope with an acousto-optical tunable filter

Luca Genchi, Andrea Bucci, Siarhei Laptenok, Carlo Liberale

Author Affiliations +

Proceedings Volume 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021; 116560D (2021) https://doi.org/10.1117/12.2577530
Event: SPIE BiOS, 2021, Online Only

Abstract

We present a high spectral resolution multiplexing acquisition mode for SRS microscopy based on a dual-beam femtosecond laser. A multi-channel Acousto Optical Tunable Filter (AOTF) generates spectral masks, given by the Hadamard matrix, by turning on and off different subsets of its 8 independent channels, corresponding to different wavelengths available within the broad bandwidth of the “pump” femtosecond laser. The SRS spectrum is retrieved by using the inverse Hadamard matrix. When additive noise is dominating, spectral measurements using the multiplexed method show the same signal to noise ratio of conventional single-wavenumber acquisitions performed with 4 times longer integration time.

Conference Presentation

Citation Download Citation

Luca Genchi, Andrea Bucci, Siarhei Laptenok, and Carlo Liberale "Hadamard-transform spectral acquisition in a broadband stimulated Raman scattering microscope with an acousto-optical tunable filter", Proc. SPIE 11656, Advanced Chemical Microscopy for Life Science and Translational Medicine 2021, 116560D (5 March 2021); https://doi.org/10.1117/12.2577530

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