Ms. Alexandra Migga Profile

Alexandra Migga

at Univ Hospital Basel

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Publications (3)

SPIE Journal Paper | 31 March 2022 Open Access

Comparative hard x-ray tomography for virtual histology of zebrafish larva, human tooth cementum, and porcine nerve

Alexandra Migga, Georg Schulz, Griffin Rodgers, Melissa Osterwalder, Christine Tanner, Holger Blank, Iwan Jerjen, Phil Salmon, William Twengström, Mario Scheel, Timm Weitkamp, Christian Schlepütz, Jan S. Bolten, Jörg Huwyler, Gerhard Hotz, Srinivas Madduri, Bert Müller

JMI, Vol. 9, Issue 03, 031507, (March 2022) https://doi.org/10.1117/12.10.1117/1.JMI.9.3.031507

KEYWORDS: Tomography, Teeth, Nerve, Spatial resolution, Hard x-rays, Cements, Synchrotrons, Imaging systems, Tissues, Data acquisition

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Purpose: Synchrotron radiation-based tomography yields microanatomical features in human and animal tissues without physical slicing. Recent advances in instrumentation have made laboratory-based phase tomography feasible. We compared the performance of three cutting-edge laboratory systems benchmarked by synchrotron radiation-based tomography for three specimens. As an additional criterion, the user-friendliness of the three microtomography systems was considered. Approach: The three tomography systems—SkyScan 2214 (Bruker-microCT, Kontich, Belgium), Exciscope prototype (Stockholm, Sweden), and Xradia 620 Versa (Zeiss, Oberkochen, Germany)—were given 36 h to measure three medically relevant specimens, namely, zebrafish larva, archaeological human tooth, and porcine nerve. The obtained datasets were registered to the benchmark synchrotron radiation-based tomography from the same specimens and selected ones to the SkyScan 1275 and phoenix nanotom m® laboratory systems to characterize development over the last decade. Results: Next-generation laboratory-based microtomography almost reached the quality achieved by synchrotron-radiation facilities with respect to spatial and density resolution, as indicated by the visualization of the medically relevant microanatomical features. The SkyScan 2214 system and the Exciscope prototype demonstrated the complementarity of phase information by imaging the eyes of the zebrafish larva. The 3-μm thin annual layers in the tooth cementum were identified using Xradia 620 Versa. Conclusions: SkyScan 2214 was the simplest system and was well-suited to visualizing the wealth of anatomical features in the zebrafish larva. Data from the Exciscope prototype with the high photon flux from the liquid metal source showed the spiral nature of the myelin sheaths in the porcine nerve. Xradia 620 Versa, with detector optics as typically installed for synchrotron tomography beamlines, enabled the three-dimensional visualization of the zebrafish larva with comparable quality to the synchrotron data and the annual layers in the tooth cementum.

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Proceedings Article | 7 October 2021 Presentation + Paper

Laboratory-based phase and absorption tomography for micro-imaging of annual layers in human tooth cementum, paraffin-embedded nerve and zebrafish embryo

Alexandra Migga, Georg Schulz, Griffin Rodgers, Melissa Osterwalder, Christine Tanner, Holger Blank, Iwan Jerjen, Phil Salmon, William Twengström, Mario Scheel, Timm Weitkamp, Christian Schlepütz, Jan Bolten, Jörg Huwyler, Gerhard Hotz, Srinivas Madduri, Bert Müller

Proceedings Volume 11840, 118400T (2021) https://doi.org/10.1117/12.2597926

KEYWORDS: Teeth, Tomography, Nerve, Spatial resolution, Synchrotrons, Data acquisition, Tissues, Synchrotron radiation

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Proceedings Article | 9 September 2021 Presentation + Paper

Impact of fixation and paraffin embedding on mouse brain morphology: a synchrotron radiation-based tomography study

Griffin Rodgers, Christine Tanner, Georg Schulz, Alexandra Migga, Timm Weitkamp, Willy Kuo, Mario Scheel, Melissa Osterwalder, Vartan Kurtcuoglu, Bert Müller

Proceedings Volume 11840, 118400P (2021) https://doi.org/10.1117/12.2595144

KEYWORDS: Brain, Bioalcohols, Tissues, Neuroimaging, Visualization, Synchrotrons, X-rays, Cerebellum

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