At present, the administration of chemotherapy drugs is largely based on physician experience and clinical guidelines. Clearly, a personalized solution would significantly benefit patients in the improvement of clinical outcomes. In this work, we used a spectroscopic approach in which a water-soluble tetrazolium salt (WST-8) assay was applied to patient-derived slice organoids. WST-8 would react with intracellular dehydrogenase resulting in the formation of formazan dye. The absorption at 450nm can be used to assess the viability of the organoids 5 days after initiation of organoid culture. With additional development, this approach may be used to improve the clinical outcome of patients undergoing chemotherapy.
In this study, we performed two-photon imaging of porcine skin and oral mucosa specimens under simulated tissue glycation. After 14 days of glycation treatment with fructose, AGEs were formed in both porcine skin and oral mucosa. The autofluorescence response of both skin and mucosa depended on the detection bandwidth with the wavelength region of 330-480 nm demonstrated the strongest detection of AGE autofluorescence.
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