Saliva presents a minimally invasive alternative medium to blood for performing diagnostics1. Microsphere sensors for ions, small organic molecules, and proteins are currently being developed and optical microarrays containing thousands of these sensors will be used for simultaneous multi-analyte analysis. The fiber bundle platform in use is 1mm in diameter and contains approximately 50,000 individually addressable 3.1μm fibers, each with an etched well capable of housing a single 3.1μm microsphere sensor. Micron-sized bead-based chemistries are produced in house, followed by deposition onto a fiber-optic bundle platform, allowing for multiplexed analysis. The ultimate goal is to develop a universal diagnostic system using saliva as the diagnostic medium. This platform will permit multiplexed analysis of a sample by integrating microfluidics with the optical arrays loaded with sensors capable of detecting relevant biomarkers associated with a wide range of disease states.
Disease states that are currently under investigation include end stage renal disease (ESRD) and Sjoegrens Syndrome (SS).
A cell array biosensor was developed, composed of thousands of individual bacteria cells expressing a reporter gene that responds to the presence of environmental pollutants. The single cell array was produced by immobilizing the cells on an optical imaging fiber. A high-density array of microwells was fabricated on the optical imaging fiber's distal face by selectively etching the individual fibers cores. Each microwell was used to accommodate a single living bacterium, allowing simultaneous monitoring of the genetic responses of all the cells in the array. The optical imaging fiber cell array platform provides a powerful tool for the fabrication of whole cell biosensors for various environmental and industrial applications.
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